YAHOO!!!!! FINALLY I GOT THIS MOTHERFU... ermm I mean, I finally got my extraction right this time with the help of my co supervisor. For the first 5 trials I failed to get any bands after the second column, but with my wonderful supervisor help, it finally appeared. I was relieved and excited this had happened. We had to alter so many things, we had to use 11 samples of my tachyzoites. ELEVEN, where as before I used only 2 or 3 tubes of my samples. Due to the very concentrated amount of parasite, I could eluted the DNA 8 times and still we got a band. While we working on the extraction, I talked to my supervisor about what happened on Friday and how I took off suddenly before 12pm. The people in my lab wondered why I left but most concluded, "Its probably stress, that is why Budleee took off". I find it quite amusing, no wonder people were just like cool with it. Anyways I am going to write down the protocol and keep it inside the parasitology lab's, lab manual which contained all the molecular work that has been done so far. At least now who ever needs to get T. gondii tachyzoites DNA from mice's peritoneal fluid, would not have to go to my ordeal for the past few days. Now all I have to do is to run PCR with my primers so I can get the DNA sequence I need for my expression. My advisor favourite word "It easy budlee, just do it one time and you will get it" WHATEVER!!!!!!!! I almost got a nervous breakdown with the extraction. Oh well, lets see, express protein, do a lethal mouse test, immunize guinea pigs, mate guinea pigs, immunized rabbits, run ELISA, run western blot, take pictures. SO EASY RIGHT!!! LIKE HELL IT IS!!!!!! So I am warning all my readers, this will not be the last time I will break down and runaway and babble on the blog when I get stressed from my advisor...This is just recollection of the moments I face in life, you may think you know me when you are reading this, but this blog is only a fragment of the many personalities that can manifest in me
Monday, June 30, 2008
Runaway2
YAHOO!!!!! FINALLY I GOT THIS MOTHERFU... ermm I mean, I finally got my extraction right this time with the help of my co supervisor. For the first 5 trials I failed to get any bands after the second column, but with my wonderful supervisor help, it finally appeared. I was relieved and excited this had happened. We had to alter so many things, we had to use 11 samples of my tachyzoites. ELEVEN, where as before I used only 2 or 3 tubes of my samples. Due to the very concentrated amount of parasite, I could eluted the DNA 8 times and still we got a band. While we working on the extraction, I talked to my supervisor about what happened on Friday and how I took off suddenly before 12pm. The people in my lab wondered why I left but most concluded, "Its probably stress, that is why Budleee took off". I find it quite amusing, no wonder people were just like cool with it. Anyways I am going to write down the protocol and keep it inside the parasitology lab's, lab manual which contained all the molecular work that has been done so far. At least now who ever needs to get T. gondii tachyzoites DNA from mice's peritoneal fluid, would not have to go to my ordeal for the past few days. Now all I have to do is to run PCR with my primers so I can get the DNA sequence I need for my expression. My advisor favourite word "It easy budlee, just do it one time and you will get it" WHATEVER!!!!!!!! I almost got a nervous breakdown with the extraction. Oh well, lets see, express protein, do a lethal mouse test, immunize guinea pigs, mate guinea pigs, immunized rabbits, run ELISA, run western blot, take pictures. SO EASY RIGHT!!! LIKE HELL IT IS!!!!!! So I am warning all my readers, this will not be the last time I will break down and runaway and babble on the blog when I get stressed from my advisor...Sunday, June 29, 2008
Petronas Family Day / Concert
AC Mizal was the host for tonight and he was his funny and witty usual self
Runaway
Post grad life..real life…… my life
Friday, June 27, 2008
Sepi, in the lab
Like the movie, its was Sepi in the lab yesterday before I watched the movie. Since everyone was not in the lab today. So I had ample time and space to do my work
Everything was there for my experiment, and I aliquot everything and sealed the original stock to be prevent contamination.
My laminar airflow is all clean and UV lighted to killed any bacteria
The centrifuges are working and the RA next door taught me what the "quick" button on the centrifuge does. (Perfect if you just want to momentarily spin your samples)
Even the thermocycler is set perfectly
Still there is not band in my gel... I spent the whole day and still no band.. I swear to god there was a band in the second column, but I think that is just wishful thinking.. I had to drag a friend from a nearby lab to confirm it.. SIGH!!! ARGHH!!!! I can't do spectophotometer because I had to buy a quartz cuvet to place the sample in.. GRRRR!!!

On the way to Pavillion to watch the movies Sepi, there was a massive JAM at Jln Sultan Ismail, I was stuck here for a 40 minutes :(.

My fuel was also running low so I had to switch off the air cond.

Hairie and I had dinner at Carl's Jr, other than the regular burger, we ordered the Chillie Cheese Fries. Hairie never had this before. This is beef, melted cheese and other spices smothered over fries.

After we both had LONG and TIRED day, its time to get messy!!! :D
Sepi, The Movie
A snapshot, the only snapshot I managed to take during the whole entire movie. Every was far to precious to only view on a VGA cameraSepi, or from what I understand means loneliness in English. Its a feeling that you get when you failed to be with your loved one, a feeling you get when you lost your loved one and the feeling where you cannot let go of your past loved even though it is over. Yes, Sepi is another film by Khabir Batia's who also made the movie Cinta. While Cinta focuses on all types of love that one can obtained, Sepi focuses on the feeling when Love is gone, lost or cannot be forgotten. As like Cinta, we follow the lives of 3 different individuals but somehow, their path crossed one day which became the pivoting moment in their life. First we have Adam, a young jolly Chef whom pastry and cakes are among of his specialty. Adam has been to so many weddings and is getting sick of being placed in the singles table. He kept seeing his friends and family member getting married one by one, deep down inside he wonders, if he will find his true love and live happily ever after like them. The second story is about Sufi, a shoemaker, and now a single dad who just lost his one true love. Sufi run's everyday to forget the past and perhaps find a future. He wonders if there is a second chance at love. The third story revolves around Imaan, a young woman in college whose passion is about theatre. She wrote a touching play that is named ironically "Sepi" which describes that feeling of loneliness if you lost or have not found your loved one. One wonders why she could feel lonely when she has a boyfriend, family, friends and even another guy who is after her.
WOW!!!! is what I felt. I mean this was a GREAT movie. I mean it grabs you and pokes you at all the right places. I never been to a cinema where its half empty (Hairie and I saw it on a weekday, who would seat so close in the front anyway unless its a weekend) but people are so quiet at certain scenes. No annoying phones or lame jokes. We laughed, we gasped, and we sat there in silence in unison. Most of the audience even sat through half the credits to see if there is anything else on or maybe they were still moved by the film. I know I was, I would shed a tear on some of the sad part and would not felt anything much on the other sad parts but even in a scene where it is not sad I would feel sad too!!!! The story was not heavy though, it was light and I think most people would understand it.
Another aspect of this movie that you should keep an eye on is the cinematography. There was 3 stories in this movie and each story has a different colour scheme. Adams story was laced with Purple and White. You can clearly see these two colours in every scene of Adam's story because this is the prominent color in Adam's life. In Sufi's story, I forgotten about their color for a while but after the movie I remembered, that it was Blue and Black. These are the colors you can see that is intentionally accent in every scene. Red was the focal point in Imaan's part of the movie. You can see this striking color in every scene. Everybody seemed to be wearing something Red and there would be at least a Red Object in every scene. The colour scheme for me matches the whole mood of each characters story perfectly. Red is the colour for passionated love that just never goes away in ones heart. Blue is the colour of peacefulness and also could be the color of depression, hence the saying "I am feeling blue", Black could also be the color of guilt that consume us inside slowly. For me Purple is like a happy colour because its kind of striking in a soft way but since to make purple there is blue hidden in the mixture, it shows a hidden sadness. White expresses purity and sincerity of a true love.
Camera angles and the soundtrack of this movie is like so good. The camera angles are so good, its as if you are there with them, from a eye level view, panoramic view, voyeurism or just from the characters point of view. For me its like the best, and makes you a part of the movie. The soundtrack.. oooh the sound track, there is a song for every feeling and every scene which matches perfectly. It lifts you mood or drag you down along with the character accordingly.
All I can say is, that this is a great movie to watch. If you don't like Malay movies, these are one of the few that will changed your mind. There is not long, mundane conversation, no poorly jokes that forces you to laughed, just a sincere view of a characters life and how it can be related to you. I give it 9/10. :D
Wednesday, June 25, 2008
That sick feeling

Tuesday, June 24, 2008
Tagged..
This is from Sam, a blogger that I recently add to my list.
1. Right now, what's at the top of your "Top 25" list on iTunes?
2. Right now, what's the first thing on your "To Do" list for today?
DNA and my weight
ARGHH!!! I am still not getting any reading on the agarose gel for my tachyzoites DNA extraction. Its getting insanely frustrating. I recent consulted with those who have been doing extraction for a long time. I found out I may need to double or triple my sample concentration. Also if I take the eluted DNA, and run PCR on it with my primers, I should be able to get a read a band on the gel, because PCR will amplified the minute DNA that is not present when running the agarose gel
This was my blood result during the first time I joined my University research on obesity. As usually all the reading are high, but it shows that I don't have diabetes or high blood pressure. However by HDL is low and my fat content is high, which is normal for me, who are fat already. I do hope all these number that should be low will be reduced by August and the number that are low should increased. huhuhu i hope so :pMonday, June 23, 2008
The Last Lunch...
Lamb curry, yum, my dad makes like the BEST lamb curry ever.
Etiquette
Etiquette, one aspect of decorum, is a code that governs the expectations of social behavior, according to the contemporary conventional norm within a society, social class, or group. Usually unwritten, it may be codified in written form. Etiquette usually reflects formulas of conduct in which society or tradition have invested
Saturday, June 21, 2008
Italian Mamak aka William
Ribena laichi and Watermelon Iceblended BOTH YUMMY!!!
First time I had roti Hawaii, basically, roti canai stuffed with egg, pineapple, chicken franks and eggs with dal, curry and one thousand island sauce. Delicious, the bread was soft and the proportion was just nice and right
Hairie had the Mee Raja Chicken, you can actually ask them for chicken only or duck only or steak only or all three of them. Apparently he loves the pandan chicken and the noodles. A very nice dish to have. This was my third time in this restaurant. We were both very hungry when we arrived here and we left feeling very full. That is bliss :D Friday, June 20, 2008
Pen is Mightier Than The Sword

Thursday, June 19, 2008
Dating Woes + In this life + In this life 2 = confusion and heartbreak
Those who knew me and H as a couple, please read everything through, sorry if some of you don't understand it but its the only way I could clarify everything up..
H, please forgive me. I am so sorry that your reputation has been tarnished for my writings. If you don't want to see me again and banished me from your life and from your world, I understand, what I have done was sheer evil and caused you much so much pain and caused your reputation on the line. Maybe this is why we cannot be together..... I am sorry..
In this life 2
We have our ups and downs... I felt like crap in the morning after subuh prayers, I just want to stay in bed and never to wake up...
I dragged my self out of bed to see the damaged to my car :(
Mid day was a bit better...
Even the weather was gloomy, but somehow this gloomy weather cheered me up :)
Prof came so I can do my job as his PA to switch off the mute button of his laptop and change the cursor icons. I just knew today that my prof had no clue how to differentiate my project from past research.. I thought he had it all figured out, but I guess I was wrong.. Sure DIE one... sigh.. they day got suckier for me.
However night time turned up to be the best time of the day. I got to chat with two people, two really good friends. One friend admitted that he was clinically depressed and took medication for it while the other one spill some beans on a common enemy. I felt light again, my mind was not blurry anymore. I mean wow! gossip and the truth has never felt so right before. Am I okay? nope I am still not okay, but do I feel better, HELL Yeah.!!!!... enough sad entries for now okay :D
Tuesday, June 17, 2008
In this life
My allergies are back and I have a headache, the whole lab is filled the paint vapour for making the ovitrap for my labmates project. I thought that would be a problem.. till...
I accidentally back up against the gate after I was rushing to meet a friend. Like my brother, we both were rushing and backed up against the gate.. The gate is now bent and wont properly closed, price to repair it. Most likely in the 4 figure range which probably increased because of the recent petrol hike.
I am not okay, I promised.. like the My chemical romance song. I am seriously not okay...I been trying to put a cheery front, a smiley face, a positive outlook of life, but this gate bending thing has been a catalyst to bring up old memories...It sounds so materialistic but this is what happened if my dad is around. Whenever something is wrong or broken he will tend to be in this tantrum, he will get ticked off at the slightest issue. I have no problem if it was just me to take the blame, however with my dad's rage, you people who are reading my blog and never met my dad would probably get a feel of his rage. Sadly I have this kind of rage too, and blow things out of proportion..Does this gate represent something.... maybe I was just suppressing and tossing all the issues that have been bothering me to the side.....Relationship that did not turn out the way I wanted it to be, weight fluctuation, my skin getting worse, my research going stagnant, and feeling trap, trap in my own house and my own life..
I want to run but I don't know where to go, I don't want to run away from my problems but I want a different perspective to it..There is just so many side of me that I could not never reveal to people...I am so tired....I need time for myself now.. to think over.. to become new..I need to cry, I need to scream, I need to sweat it out...I need myself back...
Monday, June 16, 2008
The New Guy
Here is to more students joining the parasitology lab :)
Saturday, June 14, 2008
Car owner
- Abg Awie = as far as i know, he is still car less but I remember shoving him a Proton Saga brochure as his first car
- Shafique = Proton Wira Aeroback
- Luvis = Proton Wira (1.5 gl if i am not mistaken)\
- Miss Ziana = She drives either a Gen2 her dads Waja
- Riken and Malim = both does not own a car in Aussieland, but they went on trip to a snow cover mountain with the rear tires in chain for more grip in the snow on a Ford Territory
- Inah = Imago Blue Kelisa Ezi, with a brand new shiny exhaust. She is selling the car.
- Sam = So far there has been no car related story I can remember of with Sam
- Bibik = Not sure, I know he rode a cab with a sex crazed driver and he did took pictures of his friend trying to take a 21 inch TV up to their apartment.
- Aziaya = during our time at our old Private college she used to drive a white kancil
- Sense of the insane = has a Myvi probably full of stuffed piglets dolls :p
- Veronicles - maroon Satria Neo higline automatic with 17 inch rims and white striped themed stickers
- Kingdom - A black Cerfiro
- Wahdi = Selling his red Alfa Romeo Twin Spark 146
- Sinbad = SLK (small little kancil)
- Meksinful Angel = no car related entry yet
- Adnautica = rode a Samsung car during his stay at Korea
- Mummy ita = dia membonceng hubby dia merempit ke tempat keje, tapi ada proton perdana
- Putera Rizal = Drive a brand new Mistubishi Lancer (non turbo edition)
- Red mummy = Honda City IVTEC sparkle grey
- Arch Arel = Black Proton Savvy with rainbow headlights
- Beruang madu = black Myvi
- Dr Shah = Silver Kia Spectra
- Dr Josh = Nissan X trail
- Dr Chen = Merempit but used to have proton iswara which he handed down to his brother
- Dr Malina = Mazda Premacy
- Kak Pinky = honda civic, proton wira, kancil, proton waja 1.8, and she driven a toyota estima during a trip to one of New Zealand hills.
Dating Woes
She Will Be Loved
My hamster just died, but I knew she was going to leave me soon. She had this large tumour in her abdomen. It was so big, she cannot crawl through her house or scratch her own head. So her death was expected
I buried her in our yard along with the other 2 hamster I had buried. The teddy bear hamster or the big ones live up to 3 years while the ones I have was the dwarf hamster which lives up to 2 years. I got her last year so its been almost a year since she was with us. She will be loved and will be missed. She is Ms Chu, my late dwarf hamster.
Thursday, June 12, 2008
Our house got broken into this morning around 8 am - 10 am. They cut pry open the window and cut through the grill. They probably chose our house because all 3 cars was out. The thief did not know that my younger sister and younger brother was still asleep. We suspect there was two people in this job. One of the scouted the ground floor of our house while the second one went upstairs to check the rooms. As they went from room to room, the thief tried to enter my brothers room which was locked, the thief probably slammed the door hard enough to awaken my brother. Usually my mother does that and he will stumbled getting up and saying "Ye Ma!". That is when the thief suddenly took off because there were still people in the house. Luckily no one was hurt and only a broken laptop on my dads office was taken. Luckily my digital camera and PS2 was spared because they did not noticed there was another room on the second floor. Kinda scary because this was the second time our house got broken into, the first time they sounded off the alarm while we were sleeping, this second time they snuck it when no one was at home. We going to install an alarm system in our house. However how is it, even when you installed a grill they cut it open. Even when there is people inside...Wednesday, June 11, 2008
Snow White


Yup this was stray cat that suddenly came to our house and made herself at home by entering our home. I think some one just threw her at our house. She was a pretty cat, white fur, blue eyes, very affectionate. She fell asleep on my arms. I fed her some Friskie. Sadly she came just as we were going to Terengganu. I had plans to buy a big cage for her so that we could keep her like a Hamster so that my mom will not go crazy with the cat running around. However my prediction were true, she never came back after we left her. I hope she found a new home and a new owner that will love her as much as I would have. From Cherating to the Medical Centre
We waste no time diving in, with my foolish brother doing a belly flop. Ouch! :p Padan muka hik hik
The tide is high and we saw many fishing boats docking in.
We had breakfast at this infamous kopitiam, called Hai Peng.
While enjoying our breakfast I saw this one guy talking to himself. We though he used a handsfree set, but to our amazement there was none to be found. He either used a very advanced handsfree set or he set the phone to loud speaker, but we swear we heard him only talking. Kesian, muda2 dah giler....

My older sis had Lime Twister while my younger sister got some chocolate drink thing.

I accidentally had warm milk with cinnamon. Accidently being I asked for a pina colada but was given this by mistake. My dad was already yelling at us for ordering iced drinks in the morning and he was pleased of my accidental order. Takpe lah biar lah singa happy

Of course homemade bread with coconut jam (kaya) and butter is a must in any kopitiam
Nasi dagang is a must in Terengganu okay
More peaceful serenity beaches
Hmm it was late in the afternoon, I bet all the fisherman is already at home resting or doing some other work

Keropok lekor is another must buy while staying here

We went to this Chinese seafood restaurant called Pak Su, its amusing that his Chinese name sounds like young uncle in Malay :D. My dad has been to this restaurant before I was even born.

Halal OK :D

This oyster was freaking huge, far bigger than those in Carlos. I am not sure about the price its either RM 7 or RM 9 a piece.

Stuffed crabs was good

Breaded friend squid was nice, siakap fish 3 rasa strangely taste like Assam Pedas though

Broccoli and scallops was like THE BEST. I do not like broccoli but this dish was SO GOOD!!

The butter shrimps was so so, I mean they did de veined the shrimp but the ones in Riverview taste so much better.

We ended the trip with the whole family catching a cold. My cold was so bad, on Wednesday I decided to go the UPM medical center for treatment. All the medicine above was free since I am a student. Since I got some more time after getting treatment I decided to get my teeth checked since they also have dental treatment there too. Two fillings, a checkup, consultant and a mouthwash cost only RM 12. I will definitely go here for any sickness, since its free as long I am a student :D

